American Society of Hematology

Fig. 3.

$Fig. 3. Chemokine concentrations in supernatants of PBMCs and CD8-depleted PBMCs of LTNPs stimulated with PHA (5 μg/mL) for 72 hours of culture. Open symbols, HIV isolation positivity from total PBMCs; closed symbols, HIV isolation negativity from unfractionated PBMCs, which either became positive after removal of CD8+ cells (no. 5 and 7), or remained negative (no. 4). No specific patterns of chemokine accumulation in the presence or absence of CD8+ cells were observed. Lower levels of chemokines were observed in cultures of LTNP hemophiliacs after 6 days of culture, whereas comparable levels of chemokines were produced in stimulated PBMC cultures established from uninfected donors.$

Chemokine concentrations in supernatants of PBMCs and CD8-depleted PBMCs of LTNPs stimulated with PHA (5 μg/mL) for 72 hours of culture. Open symbols, HIV isolation positivity from total PBMCs; closed symbols, HIV isolation negativity from unfractionated PBMCs, which either became positive after removal of CD8⁺ cells (no. 5 and 7), or remained negative (no. 4). No specific patterns of chemokine accumulation in the presence or absence of CD8⁺ cells were observed. Lower levels of chemokines were observed in cultures of LTNP hemophiliacs after 6 days of culture, whereas comparable levels of chemokines were produced in stimulated PBMC cultures established from uninfected donors.

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