Correlation between HIV replicative parameters and viral isolation from unfractionated PBMCs of LTNP hemophiliacs. Individuals who either were negative for HIV isolation from total PBMCs or became positive only after depletion of CD8⁺ cells showed lower levels of virus replication in plasma and PBMCs and a smaller number of proviral DNA copies than subjects who were positive for viral isolation from unfractionated PBMCs.