Calculated maximal expansion of T-TILs from FL4, FL6, FL10, FL12, and FL15 stimulated with autologous CD40-FL cells with addition of IL-2 and IFN-γ from day 3 of coculture if all T cells isolated from the tumor specimen would have been cultured only for expansion and usage in a therapeutic setting. Total number of viable cells was assessed by trypan blue exclusion test and number of CD3⁺ T cells by immunophenotyping on day 0, 7, 14, 21, and 28 of culture. Expanded cells were always greater than 97% CD3 ⁺ T cells, and therefore, expansion of total cells equals the expansion of T cells.