Calculated maximal expansion of T-TILs stimulated with CD40-FL and IL-2 with other cytokines. T-TILs (5 × 10⁶ cells) from FL4 were isolated, purified, and stimulated with CD40-FL cells in the presence of IL-2 (100 IU/mL) either alone or in combination with IL-4 (2 ng/mL), IL-7 (5 ng/mL), IL-12 (1 ng/mL), or IFN-γ (10 ng/mL). Total number of viable cells was assessed by trypan blue exclusion and the number of CD3⁺ T cells by immunophenotyping on days 0, 7, 14, 21, and 28 of culture. Expanded viable cells were always greater than 97% CD3⁺ T cells, and therefore, expansion of total cells is equal to expansion of T cells.