![Fig. 5. Effect of Tpo on megakaryocytic differentiation. (A) UT-7 cells were cultured with either Tpo alone or the combination of nocodazole and Tpo as follows. [1] The cells were cultured for 2 days in the presence of 0.1% DMSO, which was used as a solvent for nocodazole. The medium was then replaced with those containing Tpo at the concentration of 10 ng/mL, and the culture was continued for an additional 5 days. [2] The cells were treated with Tpo for 2 days, and the medium was replaced with those containing 0.1% DMSO. The culture was continued for additional 5 days. [3] The cells were treated with 50 ng/mL of nocodazole for 2 days, and the medium was replaced with those containing Tpo. The culture was continued for an additional 5 days. (B) Wright-Giemsa staining of UT-7 cells was performed after the culture with three different conditions as shown above. (Original magnification × 400). (C) The intracellular concentration of β-TG was determined by ELISA after the culture in each condition. The mean ± SD (bar) of three independent experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.3980/4/bl_0005f5.jpeg?Expires=1724146981&Signature=4V69pgTY47fkCAmIiYttSVECpgcTHkeqtguHhk9ewgrEphLfGlSJD~6DDyGqDlpgGo94LnfCWL4~o64dMb4CmLHd5Uy6XryCRHE1U8X6m1ovvGw0bwfCKrJXbaDqkxpyNibvs5Aab6~MmqJnLXNCpKSVcvB0UYLWmtWui7rAsHHU6yfYC67vYAkYs-ajDpQZf~09vrw4RzaokdemFkptuooL0mlDbGhcz8-W-p2Kcmgb05h5tx15oL8hcyyBFeE1VzYR8s0UCQGU7EX82fjQwfGsWmBcY0mpXrtwGUsqhMlEXfIG-oYIQjKj0SAk0fVwH9YkNHrjcfUq2m5-UESivg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Tpo on megakaryocytic differentiation. (A) UT-7 cells were cultured with either Tpo alone or the combination of nocodazole and Tpo as follows. [1] The cells were cultured for 2 days in the presence of 0.1% DMSO, which was used as a solvent for nocodazole. The medium was then replaced with those containing Tpo at the concentration of 10 ng/mL, and the culture was continued for an additional 5 days. [2] The cells were treated with Tpo for 2 days, and the medium was replaced with those containing 0.1% DMSO. The culture was continued for additional 5 days. [3] The cells were treated with 50 ng/mL of nocodazole for 2 days, and the medium was replaced with those containing Tpo. The culture was continued for an additional 5 days. (B) Wright-Giemsa staining of UT-7 cells was performed after the culture with three different conditions as shown above. (Original magnification × 400). (C) The intracellular concentration of β-TG was determined by ELISA after the culture in each condition. The mean ± SD (bar) of three independent experiments is shown.
