American Society of Hematology

Fig. 4.

$Fig. 4. Hyperploid UT-7 cells could produce more β-TG in response to phorbol ester than nonhyperploid cells. Counterflow centrifugal elutriation was performed as described in the Materials and Methods. (A) Representative DNA histogram of each fraction used in the subsequent experiments. (B) Wright-Giemsa staining of each fraction was performed after the culture with TPA for 5 days. (Original magnification × 400). The proportion of hyperploid cells was determined by counting more than 200 cells on cytospin specimens. Intracellular concentration of β-TG was determined by ELISA. The mean of three independent experiments is shown.$

Hyperploid UT-7 cells could produce more β-TG in response to phorbol ester than nonhyperploid cells. Counterflow centrifugal elutriation was performed as described in the Materials and Methods. (A) Representative DNA histogram of each fraction used in the subsequent experiments. (B) Wright-Giemsa staining of each fraction was performed after the culture with TPA for 5 days. (Original magnification × 400). The proportion of hyperploid cells was determined by counting more than 200 cells on cytospin specimens. Intracellular concentration of β-TG was determined by ELISA. The mean of three independent experiments is shown.

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