Fig. 1.
![Fig. 1. (A) 7C11 anti-Fas MoAb induces DNA fragmentation in RPMI 8226 MM cells. Cells were cultured in media alone and for the indicated times with 5 μg/mL of 7C11 anti-Fas MoAb. Genomic DNA was isolated, end-labeled with 0.5 mCi of [γ32-P]-dCTP, and analyzed by 1.8% agarose gel electrophoresis. MW, molecular weight markers (123-bp ladders and HindIII digest of l-DNA). (B) Quantification of anti-Fas-MoAb induced apoptosis in RPMI-8226 MM cells was performed by using propidium iodide staining technique. Cells were treated with anti-Fas MoAb for the indicated time intervals and stained with propidium iodide; percentage of apoptotic cells was analyzed by FACS. “C” represents control cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.227/2/bl_0027f1.jpeg?Expires=1724261577&Signature=srdaAJrctqWIz3~sgRaJOrkGFIcyYlvX3KINEyhREAxIcg33nsYX-v3i6u6KArbmx70sagQROKmNu0ckq1X33nnuRe~8lwp8LHghAWhjlaLmJpjH23KMWURrT62UJC~0DJ~OqQ4FeoV-8YdnIZVaVS-kd1V3OEBy9VYsdD7L4ad5xYMXUfRSxRyYq25dhuNLb7-xoXTjznUdDawK2MVEkoMwElB0cbzkAnKrCbbG-HkTn97etmc8Ran436sxYhwjkI-2ifyG5cWzKrv-~A-pL7US2NBr17L7TZ1uZeKAFDWHluxboXURsZCWnNoV~IcqgJSPLzUxnFZ3JrEJgM55EA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) 7C11 anti-Fas MoAb induces DNA fragmentation in RPMI 8226 MM cells. Cells were cultured in media alone and for the indicated times with 5 μg/mL of 7C11 anti-Fas MoAb. Genomic DNA was isolated, end-labeled with 0.5 mCi of [γ32-P]-dCTP, and analyzed by 1.8% agarose gel electrophoresis. MW, molecular weight markers (123-bp ladders and HindIII digest of l-DNA). (B) Quantification of anti-Fas-MoAb induced apoptosis in RPMI-8226 MM cells was performed by using propidium iodide staining technique. Cells were treated with anti-Fas MoAb for the indicated time intervals and stained with propidium iodide; percentage of apoptotic cells was analyzed by FACS. “C” represents control cells.
