Fig. 6.
![Fig. 6. Immunoprecipitation of cytokine-treated HUVEC by the β3 VNR antibody 10E2 and by the VNR complex antibody LM609. HUVEC were pretreated with IFN-γ and TNF-α and metabolically labeled with [35S] methionine and [35S]cysteine for the last 17 hours, as described. Immunoprecipitation of untreated HUVEC was performed on equivalent amounts of cell lysate with LM609 (lane 1) or the anti-β3 MoAb 10E2 (lane 2). TNF-α–treated (lane 3) and TNF-α +IFN-γ–treated (lane 4) HUVEC were immunoprecipitated with 10E2. The αv and β3 bands, which migrated at Mr = 140,000 and 110,000, respectively, are indicated by the arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4071/4/bl_0033f6.jpeg?Expires=1724262784&Signature=zY5zhwJNxFhUIcqp5cq89wZh9snpV1IRzsNEUQ~FuovEKpROUM2SHW6SFF16yCLxttxY9kNHuvaWVYHiQyMiVrcE3NW9dg2b7iidwi9E9Jsb09Jm40PWXwrn4y3Y8zXW9wBrkvNnj524vAZl0yFs8wtGl~yCk60KE~dqscCWf3OirkEB0RG4VCQgWJ3lewF~7qydulCc9cMuctlc3jA5JZV9hptu8PhxFayxBTpIm-G3iyIRPoIQvi7jpbc4XotSpQ1f1MJg9Ds6I7YNDBamQdr4ABxW7CXd4YPr7OiB1thfKH-wGOWAwhcarh85zet6BLeHmrPhxRhJfx5~EwV~5g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation of cytokine-treated HUVEC by the β3 VNR antibody 10E2 and by the VNR complex antibody LM609. HUVEC were pretreated with IFN-γ and TNF-α and metabolically labeled with [35S] methionine and [35S]cysteine for the last 17 hours, as described. Immunoprecipitation of untreated HUVEC was performed on equivalent amounts of cell lysate with LM609 (lane 1) or the anti-β3 MoAb 10E2 (lane 2). TNF-α–treated (lane 3) and TNF-α +IFN-γ–treated (lane 4) HUVEC were immunoprecipitated with 10E2. The αv and β3 bands, which migrated at Mr = 140,000 and 110,000, respectively, are indicated by the arrows.
