Attachment of untreated, TNF-α–treated, and TNF-α + IFN-γ–treated HUVEC to RADS-vWF in the presence of (A) vWF-A1 or (B) the GP Ibα MoAb AS-7. HUVEC were pretreated with TNF-α for 17 hours (▪) or with IFN-γ for 72 hours and TNF-α for the last 17 hours (▨), or were left untreated (□). HUVEC attachment was measured at 60 minutes as described in Materials and Methods.