Fig. 3.
![Fig. 3. SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. COS-1 cells were transiently contransfected with pTH3/WT (WT) or pTH3/R561S (R561S) and pSV–β-galactosidase plasmids. The cells were metabolically labeled with L-[35S]-methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction) and rabbit polyclonal anti–β-galactosidase (IgG fraction). The immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards and β-galactosidase is indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.183/2/bl_0016f3.jpeg?Expires=1724229913&Signature=ZmIqbrgM2n~vJQR0HQ2IzoonarsjzzibiHZVI-9gXjvjbE-KTNKLiknZ~lQ8VEwU~p8uogCUVvOAawUbObBxds7Sgs0b5llZG-crfKAp627alaQY2qEUvRn6DjP~TRI5Ry38Xslyz2uZTdI3P8TVwwJBNrvnJE4QtuwzVoTKPbClJLn~bXYMIh5DuVXjMQrcx7gCYs7wkJj2SXNjIGprU5gClE8~z9pn6~7QsG~vxZceRvKhgt6sknX6dfQK98BKlS1SKNV6GQmDOtzyWBe1pp2RsydtDcEa2vDo2m0ZFLlJmbTgO~vWH8V5Rc34lumxr5LAk1lMCnFzVvjYEuxl6A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. COS-1 cells were transiently contransfected with pTH3/WT (WT) or pTH3/R561S (R561S) and pSV–β-galactosidase plasmids. The cells were metabolically labeled with L-[35S]-methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction) and rabbit polyclonal anti–β-galactosidase (IgG fraction). The immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards and β-galactosidase is indicated.
