![Fig. 1. SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. (A) COS-1 cells were transiently transfected with pTH3/WT (WT) or pTH3/S572P (S572P) plasmids. Cells were metabolically labeled with L-[35S] methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction). Immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards is indicated. (B) Intracellular PLG purified from HepG2 cells (CE) and protein A-Sepharose beads containing adsorbed rPLG (CM) were treated with (+) or without (−) endo H as described in Materials and Methods. Each sample was boiled without a reducing agent, centrifuged, and the supernatant was subjected to 10% SDS-PAGE as described above.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.183/2/bl_0016f1.jpeg?Expires=1724228173&Signature=dOkijanVpbxKtbMGQvlfbtSnwzymWVrcOkfIR651VlzMUsqxQhklFssYpCYnte8v9N1luZWgeHUqFzv88JhMrKmTsDNqbczxCwmV3BvA6THob4pNF9IthFYKa8CsCII9SrM6xR6gfV-EQQJIfU4yRtOpaH8nyBxQIr6AsqBcrLywqBJqVmoSK7sl7fb77xgM45DUVcjO3~rJI2Ir0jWszBpKxjO3dpbymyPRmDYbobff0md3qJ2l08aCoV-3x13T~hSENbmZHUp77x20OPov2fsZzWPSwWYI~dM54CFrMJOwH6IqF07Jy8I-BvHYnBft6xaA~2Mh~XJ2vdRfy1hZQQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. (A) COS-1 cells were transiently transfected with pTH3/WT (WT) or pTH3/S572P (S572P) plasmids. Cells were metabolically labeled with L-[35S] methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction). Immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards is indicated. (B) Intracellular PLG purified from HepG2 cells (CE) and protein A-Sepharose beads containing adsorbed rPLG (CM) were treated with (+) or without (−) endo H as described in Materials and Methods. Each sample was boiled without a reducing agent, centrifuged, and the supernatant was subjected to 10% SDS-PAGE as described above.
