(A) Represents the results of differential cell morphology scores performed on the serpin 2A and vector only control transfected FDCP-Mix cells after 8 days of culturing under conditions that promote granulocyte macrophage differentiation. The results represent the pooled data of three experiments for each clone, and the sample standard deviations are shown. Primitive cells comprise blasts and early granulocytes; mature cells represent metamyelocytes, mature granulocytes, and macrophages. The mean 8-day terminal counts were 5.0 × 10⁵ cells/mL for vector only transfectants and 5.3 × 10⁵cells/mL for serpin 2A transfectants with standard deviations not exceeding 10% of the mean (not shown). Statistical comparison of the Neo clone with the highest mean number of primitive cells (Neo 7) to each of the serpin clones using a two sample t-test gave P values of <.005 for serpin clones 4 and 5 judged as a highly significant difference and <.05 for serpin 6, which is judged as significant. (B) Represents the results of the soft agar clonogenic assays performed on serpin 2A and vector only transfected FDCP-Mix cells. Clones were plated at 5 × 10³ cells/assay and the serpin 2A transfected clones were also scored at plating densities of 2 × 10³ cells/assay. Each data point was the mean result of three separate plating assays and is representative of three repeat experiments with the standard deviations shown. Statistical comparison of the neo clone with the highest clonogenic potential (Neo1) with each of the serpin 2A transfected clones using a two sample t-test gave a P value of <.005 for all clones, which is judged as a highly significant difference.