Flow cytometric analysis of cell fractions during the purification of CD34⁺Thy-1⁺ cells. CD34 versus Thy-1 expression is shown for lymphoblastoid-gated (by forward versus side scatter) cells. (A) Apheresis sample with isotype control for Thy-1. (B) Apheresis sample. (C) Reanalysis after CD34⁺ cell enrichment. (D) Reanalysis after HSCS. The percentage of CD34⁺ or CD34⁺Thy-1⁺ cells is given in the upper left or upper right quadrant of each panel, respectively.