Fig. 1.
Fig. 1. RT-PCR analysis of CSF receptors in primitive cells. Agarose-gel fractionated RT-PCR products of GM-CSF receptor α (GM-CSFR), interleukin-3 receptor α (IL-3R), β-chain of IL-3 receptor (βIL-3) and common β-chain of IL-3, IL-5 and GM-CSF receptors (βc) were transferred to nylon membranes and hybridized with internal radioactive oligonucleotides. Lane numbers refer to: (1) “no reverse transcriptase” control, (2) Rhlo lin− Ly6A/E+ c-kit+, (3) Rhhi lin− Ly6A/E+ c-kit+, (4) lin− Ly6A/E− c-kit+, (5) blank, (6) MC/9 cells, (7) FDC-P1 cells, (8) CTLL cells.

RT-PCR analysis of CSF receptors in primitive cells. Agarose-gel fractionated RT-PCR products of GM-CSF receptor α (GM-CSFR), interleukin-3 receptor α (IL-3R), β-chain of IL-3 receptor (βIL-3) and common β-chain of IL-3, IL-5 and GM-CSF receptors (βc) were transferred to nylon membranes and hybridized with internal radioactive oligonucleotides. Lane numbers refer to: (1) “no reverse transcriptase” control, (2) Rhlo lin− Ly6A/E+ c-kit+, (3) Rhhi lin− Ly6A/E+ c-kit+, (4) lin− Ly6A/E− c-kit+, (5) blank, (6) MC/9 cells, (7) FDC-P1 cells, (8) CTLL cells.

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