(A) Modulation of the CD3 ζ chain expression on PBMC co-cultured up to 28 days with parental or cytokine gene transduced LC89 cells, evaluated by immunoblotting and expressed as arbitrary O.D. units of peak area (mean of five independent experiments). (□) LC89; (⋄) LC89/IL-2; (○) LC89/IL-7; (▵) LC89/GM-CSF; (▿) LC89/TNF-α. (B) Expression at different time periods of the CD3 ζ chain, evaluated by immunoblotting, in a representative coculture experiment between PBMC and parental and cytokine gene transduced LC89 cells.