American Society of Hematology

Fig. 2.

$Fig. 2. Defensin and Lp(a) form complexes in solution. 125I-defensin (200 nmol/L) was incubated with either Lp(a) (200 nmol/L), albumin (200 nmol/L) in 200 μL PBS, or PBS alone for 60 minutes at 22°C. The mixture was loaded onto a Sephacryl S-100 column and the radioactivity and the OD at 280 nm in each 0.5-mL fraction was measured. 125I-defensin alone (⋄); 125I-defensin plus Lp(a) (▪); 125I-defensin plus albumin (□). The arrow denotes the peak OD280 for Lp(a) in the presence and absence of defensin. One experiment representative of two so performed is shown.$

Defensin and Lp(a) form complexes in solution. ¹²⁵I-defensin (200 nmol/L) was incubated with either Lp(a) (200 nmol/L), albumin (200 nmol/L) in 200 μL PBS, or PBS alone for 60 minutes at 22°C. The mixture was loaded onto a Sephacryl S-100 column and the radioactivity and the OD at 280 nm in each 0.5-mL fraction was measured. ¹²⁵I-defensin alone (⋄); ¹²⁵I-defensin plus Lp(a) (▪); ¹²⁵I-defensin plus albumin (□). The arrow denotes the peak OD₂₈₀ for Lp(a) in the presence and absence of defensin. One experiment representative of two so performed is shown.

This Feature Is Available To Subscribers Only