Gel overlay assay of CRKL binding to BCR-ABL. Lysates of 32D, 32Dp210 and 32Dp210ΔP1,P2 cells were immunoprecipitated with ABL antisera K-12, separated by SDS-PAGE, and transferred to PVDF membranes. Immunoblots were probed with (A) GST-CRKL and binding detected with a GST antibody or (B) ABL MoAb 24-21. Molecular weight markers are indicated on the left side of the panel.