Fig. 1.
Typical traces showing the effect of PTX on eosinophil aggregation induced by maximally effective concentrations of C5a, LTB4 , or PAF. Eosinophils were pretreated for 2.5 hours with vehicle (CN) or PTX (1 μg/mL, see the Materials and Methods). The cells were washed, aliquots (300 μL) were placed in a cuvette, and changes in light transmission were recorded after activation with C5a (10−7 mol/L), LTB4 (10−8 mol/L), or PAF (10−8 mol/L). The time of the addition of the agonists is shown by the arrows. The initial rapid decrease in absorbance observed in all cases is an artifact due to the addition of the agonists.
