Time-dependent adhesion of PMNs to fibrin and the effect of fibrinogen. Isolated human neutrophils (2 × 10⁶ cells/mL) were perfused over Fb-flow at a shear stress of 80 mPa for 15 minutes. The cells were diluted in HEPES buffer (▪) or in HEPES buffer containing soluble fibrinogen (5 g/L, ○). The mean ± SE of four experiments is shown. A significant time-dependent adhesion of PMNs to Fb-flow and a significant effect of soluble fibrinogen was observed (repeated-measures ANOVA, P < .01).