PMN adhesion to surface-bound platelets and fibrin. Isolated resting neutrophils (2 × 10⁶ cells/mL HEPES buffer) were perfused at different shear stresses to determine adhesion to Fb-flow surface (•), Fb + platelets (▪), or ECM-bound platelets (○). (A) Total number of surface-interacting PMNs per mm2 measured after 5 minutes of perfusion (mean ± SE, n = 4). Fb-flow showed significantly less adhesion as determined by repeated-measures ANOVA (P < .05). (B) Rolling neutrophils calculated as a percentage of the total number of surface-interacting cells. ECM-bound platelets induced more rolling compared with the other surfaces as determined by repeated-measures ANOVA (P < .05).