American Society of Hematology

Fig. 2.

$Fig. 2. Immunoblot analysis of murine neutrophil-enriched BM. Neutrophil-enriched BM preparations were obtained from wild-type (WT) mice, X-CGD mice (CGD), and X-CGD mice 13 to 15 weeks after transplantation with MSCV-m91Neo–transduced X-CGD BM. Cellular proteins were extracted from either nonadherent BM cells (lanes 1 through 9) or a neutrophil-enriched Percoll fraction of marrow cells (lanes 10 through 13), and analyzed for expression of gp91phox, p22phox, and p47phox by immunoblotting with specific antibodies. Ten micrograms of extracted cellular protein was loaded for each sample. The immunoreactive bands in the control X-CGD samples probed with the gp91phox antibody are presumed to represent proteins that bind nonspecifically because they are not detected with other gp91phox-specific antibodies.$

Immunoblot analysis of murine neutrophil-enriched BM. Neutrophil-enriched BM preparations were obtained from wild-type (WT) mice, X-CGD mice (CGD), and X-CGD mice 13 to 15 weeks after transplantation with MSCV-m91Neo–transduced X-CGD BM. Cellular proteins were extracted from either nonadherent BM cells (lanes 1 through 9) or a neutrophil-enriched Percoll fraction of marrow cells (lanes 10 through 13), and analyzed for expression of gp91^phox, p22^phox, and p47^phox by immunoblotting with specific antibodies. Ten micrograms of extracted cellular protein was loaded for each sample. The immunoreactive bands in the control X-CGD samples probed with the gp91^phox antibody are presumed to represent proteins that bind nonspecifically because they are not detected with other gp91^phox-specific antibodies.

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