Figure 4.
Figure 4. DOCK2 factors in Wnt5a-induced activation of Rac1/2, but not RhoA. (A) Immunoblot analysis of lysates prepared from freshly isolated primary CLL cells transfected 72 hours previously with control siRNA or siRNA targeting DOCK2, then treated without (−) or with (+) Wnt5a; expression of DOCK2, total Rac1/2, RhoA, and activated Rac1/2, RhoA was measured, as indicated on the left (representative of 3 patients). The numbers between 2 lanes are ratios of band IOD of GTP-Rac1/2 or GTP-RhoA vs total Rac1/2 or RhoA, respectively. (B) Immunoblot analysis of lysates prepared from MEC1-ROR1 cells transfected 72 hours previously with control siRNA or siRNA targeting DOCK2, then treated without (−) or with (+) Wnt5a; expression of DOCK2, total Rac1/2, RhoA, and activated Rac1/2, RhoA was measured, as indicated on the left. The numbers between 2 lanes are ratios of band IOD of GTP-Rac1/2 or GTP-RhoA vs total Rac1/2 or RhoA, respectively.

DOCK2 factors in Wnt5a-induced activation of Rac1/2, but not RhoA. (A) Immunoblot analysis of lysates prepared from freshly isolated primary CLL cells transfected 72 hours previously with control siRNA or siRNA targeting DOCK2, then treated without (−) or with (+) Wnt5a; expression of DOCK2, total Rac1/2, RhoA, and activated Rac1/2, RhoA was measured, as indicated on the left (representative of 3 patients). The numbers between 2 lanes are ratios of band IOD of GTP-Rac1/2 or GTP-RhoA vs total Rac1/2 or RhoA, respectively. (B) Immunoblot analysis of lysates prepared from MEC1-ROR1 cells transfected 72 hours previously with control siRNA or siRNA targeting DOCK2, then treated without (−) or with (+) Wnt5a; expression of DOCK2, total Rac1/2, RhoA, and activated Rac1/2, RhoA was measured, as indicated on the left. The numbers between 2 lanes are ratios of band IOD of GTP-Rac1/2 or GTP-RhoA vs total Rac1/2 or RhoA, respectively.

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