Mice reconstituted with bone marrow cells from KMUKO mice have shorter survival and accumulation of myeloblasts in bone marrow. (A) Survival curve of mice transplanted with 5 × 106 bone marrow cells from KM or KMUKO mice. (B) Spleen weight 5-7 weeks after transplant. (C) Blood cell counts 4 weeks after transplant. Frequency of LSK cells, CMPs, GMPs, and MEPs (D) and Ly6C Ly6G CD11b+ cells (E) in KM- and KMUKO-recipient mice. (F) Analysis of bone marrow cells using Lin (Ter119, B220, CD3, Mac1), CD34, cKit, CD16/32 (FcγRII/III), and Ly6G antibodies. Lin− cells were separated into CD34+cKit+ and CD34−cKit− cells, and expression of CD16/32 and Ly6G was analyzed. KMUKO-recipient mice had increased numbers of myeloblasts (CD34+cKit+ fractions 1 and 2) and decreased numbers of segmented mature neutrophils (CD34−cKit− fraction 6), indicating a block at the myeloblast stage of maturation. *P < .05, **P < .01, ***P < .001, ****P < .0001, unpaired 2-tailed Student t test. PLT, platelets; RBC, red blood cells; WBC, white blood cells.
