Differential effects of antifibrinolytic drugs on joint vs tail bleeding. Joint bleeding after knee injury in FVIII^−/− BALB/c mice (n = 9-14) (A) or anti-FVIII antibody–treated (0.25 mg/kg) WT C57Bl/6J or TAFI^−/− C57Bl/6J mice (n = 9-15) (B) upon treatment with recombinant human FVIII (rhFVIII; 200 IU/kg), TXA, amiloride, or anti-uPA antibody (10 mg/kg). Bleeding was determined by measuring Hct at day 2 (D2) postinjury. (C) Tail bleeding in FVIII^−/− BALB/c mice (n = 4-12) upon treatment with rhFVIII (200 IU/kg), TXA, or anti-uPA antibody (10 mg/kg). Bleeding was determined by measuring the mouse weight-normalized blood loss (µL/g) after tail resection for 20 minutes. (D) Bleeding determined by Hct at 24 hours after tail resection in FVIII^−/− BALB/c mice (n = 4-12) upon treatment with rhFVIII (200 IU/kg), TXA, or anti-uPA antibody (10 mg/kg). Kruskal-Wallis test was used followed by Dunn’s post hoc multiple comparison test to compare experimental mice. *P < .05, **P < .01, ****P < .0001. ns, not significant.