Defective TAFI activation after knee joint injury in FVIII^−/− mice. (A) Representative examples (n = 2) of immunoblot signals (originating from the same blot with equal exposure) of zymogen TAFI levels precipitated from plasma samples 2 days after injury of FVIII^−/− BALB/c without or with administration of recombinant human FVIII (rhFVIII; 200 IU/kg) or WT C57Bl/6J mice with anti-FVIII antibody (GMA-8015; 0.25 mg/kg) alone or in combination with anti-TAFI antibody (MA-TCK26D6; 3.75 mg/kg). (B) Quantification of plasma zymogen TAFI levels 2 days after injury by immunoblot after pull down in FVIII^−/− mice without (n = 9) or with (n = 4) administration of rhFVIII (200 IU/kg) or in WT mice with anti-FVIII antibody (GMA-8015; 0.25 mg/kg) alone (n = 9) or in combination with anti-TAFI antibody (MA-TCK26D6; 3.75 mg/kg; n = 4). Kruskal-Wallis test was used followed by Dunn’s post hoc multiple comparison test to compare experimental mice. *P < .05, **P < .01. ns, not significant.