hif-1α and hif-2α morphants show reduced numbers of cmyb:EGFP⁺;kdrl:mCherry⁺cells in the VDA. (A-C) Maximal intensity projections of time-lapse confocal images of 36 hpf Tg(cmyb:EGFP);Tg(kdrl:mCherry) control and hif-1α and hif-2α morphants. Arrows point to cmyb:EGFP⁺;kdrl:mCherry⁺ cells in the VDA, and arrowheads point to cmyb:EGFP⁺;kdrl:mCherry⁺ emerging HSCs. Scale bar, 50 μm. (D) Quantification of cmyb:EGFP⁺;kdrl:mCherry⁺ cells in the VDA and emerging HSCs in a 10-somite-long trunk area in control and hif-1α and hif-2α morphants (lateral views) (n = 10 embryos from 3 different clutches). ***P < .001; ****P < .0001. ns, not significant (Student t test).