hif-1α^−/−and hif-2α^−/−exhibit decreased runx1 and cmyb expression. (A-L) Brightfield images of WISH for runx1, cmyb, and runx1/cmyb expression in (A-C and G-I) WT sibling, (D-F) hif-1α^−/−, and (J-L) hif-2α^−/− embryos (stages shown in the figure). The VDA region is outlined and shown at higher magnification in C, F, I, and L (lateral views). Scale bar, 100 μm. (M-O) Quantification of runx1 (A, D, G, and J), cmyb (B, E, H, and K), and runx1/cmyb (C, F, I, and L) WISH results, showing the percentages of embryos with WT and decreased expression in the genotypes listed. n/n, number of embryos showing representative phenotype/total number of embryos examined.