Anti-LBD mAbs, but not others, induce shear-dependent signaling. (A) Illustration of GPIb-IX domains and differential effects of mAbs. Locations of mAb epitopes are noted by arrowheads. (B) Graphs of percentage of positive events for β-galactose, phosphatidylserine, and P-selectin exposure in human (top) and hTg mouse (bottom) PRP treated with control IgG, AN51, NITA, SZ2, 6B4, or 11A8 as indicated under static or sheared conditions. (C) Graph of P-selectin exposure in hTg mouse PRP treated with control IgG, AK2, 6B4-Fab, or 6B4 under static or sheared conditions. (D) Graph of P-selectin exposure in human PRP treated with control IgG, FMC25, RAM.1, or 5G6 under static or sheared conditions. For all graphs, gray bars represent 0 dyn/cm2 and red bars represent 30 dyn/cm2. *P ≤ .05; **P ≤ .01; ***P ≤ .001. n.s., not significant.
