Increased chromatin accessibility in Asxl1^Y588XTg cKit⁺cells. (A) RNA-seq heat map depicting the 500 most significantly dysregulated genes (FDR) in BM cKit⁺ cells isolated from Asxl1^Y588XTg and WT mice (n = 5 each genotype). (B) Gel electrophoretic analyses of DNA recovered from MNase-digested nuclei of 32D cells (+) with either ASXL1^aa1-587 or empty vector (EV) nontreated cells were used as control (−). (C) Heat maps of the genome-wide ATAC-seq signal around TSS (±3000 bp). (D) GSEA plots depicting HSC and myeloid pathway gene enrichment. (E) Violin plots displaying chromatin accessibility around TSS for the genes in the 3 pathways indicated (also see panel C). Data were normalized to WT, percentage of increase in normalized reads and P values (unpaired Student t test) are indicated in the right of the plots. (F) ATAC-seq signal tracks around the Hoxa9 and Hoxb2 genes. Increased accessibility was present in Asxl1^Y588XTg cKit⁺ cells.