Association of redox sensitivity with antioxidant cell systems. (A) Patients were grouped on the basis of high and low H2O2-sensitive values, referred to the median signal values of the combined sum of pSYK, pERK1/2, and pp38, each measured as log2-fold change. Comparisons between high and low H2O2-sensitive groups for relative gene expression of CAT (catalase; n = 26), glutathione peroxidase (GPX; n = 26), SOD1 (Cu,Zn-superoxide dismutase, CuZnSOD; n = 26), SOD2 (Mn-superoxide dismutase, MnSOD; n = 26), reduced-thiol levels (detected by ThiolTracker probe; n = 18), and cell antioxidant capacity (n = 8) were performed using the Student t test. Data are expressed as mean ± SEM. *P ≤ .05; **P ≤ .01. (B) Flow cytometry histograms showing protein expression of catalase, GPX, CuZnSOD, and MnSOD in a representative low H2O2-sensitive sample (n = 3) and a representative high H2O2-sensitive sample (n = 3). Dashed lines represent autofluorescence signals.
