HO-1 expression is increased in the liver and decreased in Ter119⁺cells of the bone marrow and the spleen of Th3/⁺mice. (A) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of HO-1 mRNA expression in the liver of Wt and Th3/⁺ mice. The results are presented as fold of change relative to the Wt sample (n = 3). (B) Western blot analysis of HO-1 protein expression in the liver of Wt and Th3/⁺ mice (n = 3). (C) Representative pictures (20× magnification; scale bar, 200 µM) of immunohistochemical staining against HO-1 in the liver from Wt and Th3/⁺ mice. (D) qRT-PCR analysis of HO-1 mRNA expression in Ter119⁻ and Ter119⁺ cell populations isolated from the bone marrow of Wt and Th3/⁺ mice. The results are presented as fold change relative to Wt Ter119⁻ cells (n = 3). (E) Western blot analysis of HO-1 protein expression in Ter119⁺ bone marrow samples (n = 3). (F) Total heme levels in Ter119⁻ and Ter119⁺ cells isolated from the bone marrow of Wt and Th3/⁺ mice (n = 6). (G) Western blot analysis of HO-1 protein expression in Ter119⁺ spleen samples (n = 3). (H) Total heme levels in Ter119⁻ and Ter119⁺ cells isolated from the spleen of Wt and Th3/⁺ mice (n = 6). *P < .05, ***P < .001.