The sensitivity to antigen of γδ20 TCR-plus-CRISPR–transduced CD8⁺ cells is higher by several orders of magnitude than the sensitivity of CD8⁺ cells transduced only with γδ20 TCR. (A) The sensitivity to the titrated antigen HMBPP was measured either by CD69 mobilization (Jurkat) or MIP-1β production (transduced T cells and T-cell clone) after overnight incubation with the antigen. CD69 mean fluorescence intensity or MIP-1β concentration was normalized by subtracting the values of unstimulated cells, and assuming the maximum value as 100%. The EC₅₀ values were calculated in GraphPad Prism software by nonlinear regression curve fitting. (B) The 50% effective concentration (EC₅₀) represented as molar concentration of antigen and fold change. Representative data of 2 independent experiments carried out in duplicate are shown.