miR-17-92 regulates B-cell differentiation and function in cGVHD. B6 to BALB/c murine model of cGVHD was set up as described in Figure 1. Sixty days after BMT, data from 1 representative mouse of each group (A) and mean percentage of GL-7⁺Fas⁺ GC B cells on gated H2K^b+B220⁺ cells, and B220⁻CD138⁺ plasma cells on gated H2K^b+ cells are shown in spleen (B). (C) Sera from whole blood were taken for enzyme-linked immunosorbent assay (ELISA) measuring anti-dsDNA IgG and IgG2c autoantibodies as described in “Materials and methods.” (D) Mean fluorescence intensity (MFI) of MHC-II (IA^b) and CD86 expression are shown on gated H2K^b+B220⁺ in spleen (N = 4-5 mice per group shown). Data shown are the representative of 3 individual experiments with a cumulative total of 15 to 20 mice per group. (E) Serum IL-10 levels in recipients were measured using the CBA kit 60 days post-BMT (N = 4-5 mice per group shown). *P < .05; **P < .01; ***P < .001.