miR-17-92 regulates CD4 T-cell differentiation in cGVHD. BMT was carried out as described in Figure 1. Sixty days after BMT, recipient spleens were collected and mononuclear cells were isolated and subjected to fluorescence-activated cell sorting (FACS) staining. Data from 1 representative mouse of the group (A, top) and mean percentage of Tfh cells (PD-1^hiCXCR5⁺ cells on gated H2K^b+TCRvβ⁺CD4⁺Foxp3⁻ T cells) and ratio of Tfr to Tfh cells (ratio of Foxp3⁺ to Foxp3⁻ cells gated on H2K^b+TCRvβ⁺CD4⁺PD-1^hiCXCR5⁺ cells) are shown (B, top). Data from 1 representative mouse of each group (A, bottom) and mean percentages of IFNγ⁺, IL-4/5⁺, and IL-17⁺ cells are shown on gated H2K^b+ TCRvβ⁺CD4⁺ T cells (B, bottom) (N = 4-5 mice per group shown). Data shown are the representative of 3 individual experiments with a cumulative total of 15 to 20 mice per group. *P < .05; **P < .01.