miR-17-92 regulates CD4-T cell infiltration and antibody deposition in skin. BMT was performed as described in Figure 1. Recipient skin was harvested at day 60 after transplantation and processed for hematoxylin and eosin (H&E), Masson trichrome stain for collagen (blue area), immunohistochemistry staining for CD4 (brown), and immunofluorescence staining for IgG (green area) as described in supplemental Methods. (A) Representative images from 3 individual experiments are shown and black arrows point to CD4⁺ cells (original magnification ×200). (B) Percentage of area with collagen deposition was analyzed using ImageJ. (C) CD4 cell infiltration was quantified by calculating the average ratio of CD4⁺ cells to total cells in a 100-mm² field of view under microscope from 3 representative fields. (D) IgG intensity in skin was analyzed using ImageJ software and mean fluorescence intensities are shown from 3 representative fields. N = 4-5 mice per group. **P < .01; ***P < .001. DAPI, 4′,6-diamidino-2-phenylindole.