Genome-wide methylation analysis. (A) Unsupervised hierarchical clustering based on methylation profiles of patients with JMML (n = 94) or NS/MPD (n = 12) and repository data from normal CD34⁺ (n = 5) and AML CD34⁺CD38⁻ samples (n = 14). (B-C) Gene set enrichment analysis. (D) OS and (E) TFS of patients with JMML according to the methylation profiling-based classification. Patients with NS/MPD were excluded from survival analyses. FDR, false discovery rate; HSC, hematopoietic stem cell; NES, normalized enrichment score.