Effect of crizotinib on colony formation by JMML cells with ALK/ROS1 fusion. (A-C) Effect of crizotinib (ALK/ROS1/MET inhibitor) on colony formation by RANBP2-ALK+ CD34+ cells. A total of 1 × 103 CD34+ cells from a patient with RANBP2-ALK was cultured for 2 weeks with or without cytokine-supplemented culture media in the presence or absence of the indicated amount of crizotinib. (A) Microscopic appearance of colony-forming unit, granulocyte-macrophage (CFU-GM) colonies. (B-C) Numbers of colonies (B) with and (C) without cytokines. (D-F) Effect of crizotinib on colony formation by DCTN1-ALK+ CD34+ cells. (D) Microscopic appearance of CFU-GM colonies. (E-F) Numbers of colonies (E) with and (F) without cytokines. BFU-E, burst-forming unit erythroid. †P < .01.
