Lyzs-mTOR KO mice show normal percentage and cell number of macrophages. (A) GMPs, monocytes, neutrophils, and macrophages were sorted from bone marrow, spleens, and the peritoneal cavity of WT and Lyzs-mTOR mice, respectively; p-S6 expression was analyzed by western blot. (B) Representative fluorescence-activated cell sorter analysis of spleens, peritoneal cavity, and colon macrophages for staining CD11b⁺F4/80⁺ was shown. The percentages (C) and cell numbers (D) of CD11b⁺F4/80⁺ cells in peritoneal cavity, spleen, and colon from WT and Lyzs-mTOR KO mice were assayed. Data are representative of 3 independent experiments.