Inhibition of Notch signaling promotes megakaryopoiesis from CB CD34⁺cells. (A) Representative FACS analysis of the percentage of MKs (CD41⁺CD42⁺) derived from CB CD34⁺ cells with addition of 10 μM GSIs (RO4929097 or DAPT) or DMSO (control). (B) Bar graph depicting fold changes in the cell numbers of MKs derived from CB CD34⁺ cells with addition of 10 μM GSIs (RO4929097 or DAPT) or DMSO (control). Data are shown as the means ± SEM, n = 3. *P < .05. (C) The number of CFU-MK colonies from 7500 cells on day 14 derived from CB CD34⁺ cells with addition of 10 μM GSIs (RO4929097 or DAPT) or DMSO (control). Data are shown as the means ± SEM, n = 3. *P < .05; **P < .01.