DAC treatment upregulates NK-inducing ligands on THP-1 cells in vivo. (A-B) THP-1–bearing mice were treated with DAC (1.25 mg/m²) for 5 consecutive days. One week after the start of treatment, mice were euthanized and bone marrow cells were isolated for ex vivo analysis of THP-1 cells. Data obtained from 5 individual mice per treatment group were pooled to reach enough events by FCM (500-900 single and viable THP-1 cells acquired per test). The relative expression level of NKG2D and DNAM-1 ligands, as well as death receptors, are depicted in panel A, and overlay plots with mean fluorescence intensities are shown in panel B. Iso, isotype control.