Gene expression profiles of cCLPs and CLPs ex vivo. (A) Heat map view of representative gene expression profiles on transcription factors and receptors in cCLPs (in vitro) and CLPs ex vivo (n = 4). (B) Statistical analyses of representative gene expression of transcription factors (top), surface markers (middle), Notch family and cytokine receptors (lower left), and chemokine receptors (lower right) associated with the hematopoietic cell development measured by RNA-seq in cCLP (red bars) or CLP ex vivo (blue bars). Read counts after normalization were used as gene expression (see “Materials and methods”). Error bars indicate mean (n = 4) ± standard deviation. (C) Quantitative reverse transcription polymerase chain reaction analysis of CLP- or B-cell–related gene expression (n = 4). Relative quantities (RQ) of the indicated genes normalized to the expression of Gapdh are shown. (D) Two-dimensional principal component analysis of normalized whole gene expression profiles in cCLPs (in vitro, n = 4, in red), freshly isolated CLPs (ex vivo, n = 4, in blue), and other hematopoietic cells (in green). preBI, precursor BI (B220⁺CD19⁺ckit⁺IL7R⁺D_HJ_H/D_hJ_H-rearranged). n.s., not significant.