P4HA2 promotes the growth of B lymphoma. Suppression of P4HA2 inhibits proliferation of B-lymphoma cells. Growth assays were carried out with SU-DHL-4 (A), SU-DHL-6 (B), and NU-DUL-1 (C) cells of P4HA2 knockdown. Points, mean (n = 6); bar, SD; ***P < .001. (D-F) Suppression of P4HA2 inhibits proliferation of B-lymphoma cells in vivo. The resected tumors (D) from individual nude mice. Eight mice were used in each group. The tumor volume (E) of the xenografts. Points, mean (n = 8); bar, SD; ***P < .001. The tumor weight (F) of the xenografts after dissection. Scatter plot, mean (n = 8); bars, SD; ***P < .001. (G) P4HA2 improves B-lymphoma cell proliferation depending on its enzyme activity. SU-DHL-4 cells were stably transfected with P4HA2 (P4), enzyme inactivation (P4-M), and the control (Vehicle) by lentivirus, respectively. Cell growth assay was performed as above. Points, mean (n = 6); bar, SD; *P < .05; ***P < .001. (H) P4HA2 facilitates Erk1/2 phosphorylation depending on its enzyme activity. Phosphorylation of Erk was detected after PMA stimulating as depicted in Figure 3J. (I-K) P4HA2 promotes B-lymphoma cell proliferation in vivo depending on its enzyme activity. The resected tumors (I) from individual nude mice. The tumor volume (J) of the xenografts. Points, mean (n = 8); bar, SD; ***P < .001. The tumor weight (K) of the xenografts after dissection. Scatter plot, mean (n = 8); bars, SD; ***P < .001.