Aberrant platelet production and platelet receptor expressions in Csk KO mice. (A) Platelet lysates were blotted for the indicated proteins. (B) Platelet counts. (C) Percentage of reticulated platelets determined by thiazole orange⁺αIIb⁺ cells in blood. See also supplemental Figure 2A. (Di) Median fluorescence intensity (MFI) measured in αIIb⁺ cells alone or αIIb⁺ cells costained for the indicated proteins in blood (n = 5-6 mice per genotype). See also supplemental Figure 3. (Dii) Platelet lysates were blotted for the indicated proteins. (Ei) Platelet counts of pre-/postinjection of anti-GPIbα antibody (1.5 μg/g body weight). (Eii) The rate of platelet recovery determined by a proportionate slope from linear trend lines between days 3 and 7 from panel Ei (n = 7-8 mice per genotype). See also supplemental Figures 4-6. Asterisks refer to significant difference compared with WT (*P < .05, ***P < .001; 1-way ANOVA with Tukey’s test in B-D or 1-way ANOVA with Dunnett’s test; vs WT in E); data represent mean ± SEM.