(A) Cartoon of the sequencing strategy, demonstrating targeting the IgH gene rearrangements. Multiple primers complementary to various V and J sequences are simultaneously used, and in this example, primers specific to V4 and JB bind and can form a PCR VDJ rearrangement product. The amplified rearrangements are sequenced and bioinformatics approaches are used to identify the dominant clones that will subsequently be tracked for the MRD assay. (B) Kaplan-Meier estimates of EFS for standard-risk group patients who were MRD positive by HTS at any level vs MRD negative. See Figure 4A in the article by Wood et al that begins on page 1350.