Figure 5.
Figure 5. The platelet deposition defect of Tlr2−/− mice is microbiota dependent. (A) VWF level in PPP of GF WT compared with GF Tlr2−/− mice (5 mice per group). (B) VWF multimer composition in PPP from GF WT and GF Tlr2−/− mice (3 mice per group). (C) VWF mRNA expression in the livers (7 mice per group) and the lungs (7 mice per group) of GF WT and GF Tlr2−/− mice. (D) Deposition of DCF, 5- (and 6) carboxy-2’,7’-dichlorofluorescein diacetate (carboxy-DCFDA)-stained platelets (green) to the ligation-injured common carotid artery in GF WT (left) or GF Tlr2−/− (right) mice 30 minutes after transient ligation (7-9 mice per group). Scale bar, 200 µm. Mean ± SEM; independent-sample Student t tests or repeated measurement ANOVA (mixed model). n.s., not significant.

The platelet deposition defect of Tlr2−/−mice is microbiota dependent. (A) VWF level in PPP of GF WT compared with GF Tlr2−/− mice (5 mice per group). (B) VWF multimer composition in PPP from GF WT and GF Tlr2−/− mice (3 mice per group). (C) VWF mRNA expression in the livers (7 mice per group) and the lungs (7 mice per group) of GF WT and GF Tlr2−/− mice. (D) Deposition of DCF, 5- (and 6) carboxy-2’,7’-dichlorofluorescein diacetate (carboxy-DCFDA)-stained platelets (green) to the ligation-injured common carotid artery in GF WT (left) or GF Tlr2−/− (right) mice 30 minutes after transient ligation (7-9 mice per group). Scale bar, 200 µm. Mean ± SEM; independent-sample Student t tests or repeated measurement ANOVA (mixed model). n.s., not significant.

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