Impaired deposition of Tlr2-deficient platelets to laminin coatings is dependent on reduced plasma VWF levels and hepatic VWF synthesis in Tlr2^−/−C57BL/6 mice. (A) Relative VWF mRNA expression in the livers and lungs of WT, Tlr2^−/−, and Tlr4^−/− mice (7-9 mice per group). (B) VWF level in PPP of WT and Tlr2^−/− mice (8 mice per group). (C) VWF multimer composition in PPP of WT and Tlr2^−/− mice (4 mice per group). (D) FVIII mRNA levels in primary mouse liver endothelial cells of WT and Tlr2^−/− mice (5 mice per group). (E) VWF mRNA levels in primary mouse liver endothelial cells of WT and Tlr2^−/− mice (5 mice per group). (F) Static laminin deposition of DCF-stained, washed platelets (green) of WT or Tlr2^−/− mice after 30 minutes of incubation with WT or Tlr2^−/− plasma. Representative images (left) and quantification (right); quadruplicate measurements (3 mice per group). Scale bar, 100 µm; mean ± SEM; independent-sample Student t tests or ANOVA with Tukey post hoc test or Kruskal-Wallis test, *P < .05. EC, endothelial cell; n.s., not significant.