Reduced plasmatic VWF levels, hepatic synthesis, and platelet deposition in GF C57BL/6 mice. (A) Deposition of DCF, 5- (and 6) carboxy-2′,7′-dichlorofluorescein diacetate (carboxy-DCFDA)-stained platelets (green) to the ligation-injured common carotid artery in CONV-R WT (top) and GF WT mice (bottom) 30 minutes after transient ligation. Images of representative experiments are shown. Scale bar, 200 µm (10 mice per group). (B) VWF levels in PPP of CONV-R WT and GF WT mice (5-7 mice per group). (C) FVIII levels in PPP of CONV-R WT and GF WT mice (5-8 mice per group). (D) ADAMTS13 levels in plasma of CONV-R WT and GF WT mice (3 mice per group). (E) Relative VWF mRNA expression in the livers (4-5 mice per group), lungs (6 mice per group), and bone marrow (5 mice per group) of CONV-R WT compared with GF WT mice. (F) Relative FVIII mRNA expression in the liver (7 mice per group). (G) Deposition of DCF-stained, washed platelets (green) from CONV-R WT or GF WT mice to laminin. Representative images, scale bar, 100 µm; quadruplicate determination (4 mice per group). (H) Deposition of washed platelets from CONV-R WT and GF WT mice to laminin after 30 minutes of incubation with WT or Vwf^−/− plasma; quadruplicate measurements (4 mice per group). (I) Deposition of washed platelets from Vwf^+/+ and homozygous Vwf^−/− mice to laminin after 30 minutes of incubation with Wilfactin ± α-RGD; quadruplicate determination (4 mice per group); scale bar, 100 µm. Mean ± SEM, repeated measurement ANOVA (mixed model); independent-sample Student t tests or ANOVA with Tukey post hoc test, *P < .05. n.s., not significant.