CD207 expression in lung CD11c⁺cells driven by KRAS^G12D. (A) Polymerase chain reaction (PCR) detection of the recombined KRAS^G12D (Lox-G12D) allele in sorted hematopoietic populations from the AdCre/KRAS^G12D lung. The recombined Lox-G12D allele is clearly detected in CD45⁺CD11b^lowCD11c⁺ cells (population 2) at a similar level to the KRAS^WT (WT) allele. (B) Left, Giemsa staining of sorted CD45⁺CD11b^lowCD11c⁺ cells. This population is composed of small cells showing basophilic cytoplasm with relatively high nuclear/cytoplasmic (N/C) ratios, and larger macrophage-like cells. Scale bar, 10 μm. Right, confocal imaging of enriched CD11c⁺ cells stained for CD207 (green) and MAC2 (red). Reticular CD207 staining in cytoplasm and variable levels of membranous/cytoplasmic MAC2 staining detected in most cells. Scale bar, 10 μm. (C) CD207 expression in the AdCre/KRAS^G12D lung detected by IHC (top) and immunoblotting (bottom). Scale bars (top panels), 62.5 μm. DIC, differential interference contrast; SSC, side scatter.