Quantitative reverse-transcriptase polymerase chain reaction analysis of LOX in peripheral blood platelets and megakaryocytes differentiated from control HSs and patients with PMF. (A) Equal numbers of cells were evaluated in the experimental groups, and values were normalized to GAPDH messenger RNA (mRNA) and expressed relative to HSs. Data are expressed as mean ± standard deviation (SD) of 10 samples from patients with PMF and 5 from HSs. *P < .05, **P < .01. (B) An equal number of washed platelets from peripheral blood of HSs or patients with PMF were plated on pepsinated monomeric type I collagen (PSCI) or triple helical native acid soluble type I collagen (ASCI). Adherent platelets were fixed and stained with anti β-actin antibody (red). Scale bar, 50 µm. (C) Platelets (plt) were counted and expressed as platelets per mm². Spread area was measured using Olympus LCmicro software and expressed as pixels. Data of adhesion to monomeric collagen (top bars) or fibrillar collagen (lower bars) are expressed as mean ± SD. *P < .05. Supplemental Methods provides additional details on procedures and statistics.