Figure 1.
Figure 1. Robust engraftment of CMML patient samples into NSGS mice. (A) Schema of method for engraftment of human CD34+ (hCD34+) cells from the BM of CMML patients into sublethally irradiated NSGS mice and serial transplantation. Each patient sample was injected into 1 NSGS-recipient mouse. (B) Clinical and genetic characteristics of CMML patient samples successfully engrafted along with number of cells transplanted per recipient mouse and chimerism of hCD45+ cells in BM and spleen of NSGS mice, French-American-British (FAB) classification. (C) Kaplan-Meier survival curve of recipient NSGS mice engrafted with CD34+ cells from CMML patients (n = 8). (D) White blood cell (WBC) count, hemoglobin (Hb), and platelet (PLT) count of recipient NSGS mice at initial bleed (2 weeks posttransplantation) and at time of moribund state (the mean value is represented by the line inside the box ± standard deviation represented by lines above and below the box; *P < .05, **P < .01; number of individual mice is represented in the figure). (E) Cytomorphology of primary patient PB and BM (top) and corresponding NSGS-recipient mouse BM xenografted from the same patient (bottom). Black arrows indicate dysplastic neutrophils; red arrow indicates binucleated erythroid precursor; scale: 50 μm. (F) Immunophenotype of a representative CMML patient BM (left column) and tissues from a corresponding recipient mouse xenografted from the same patient (the percentages listed represent the percent of cells within the gate described above each plot). (G) Hematoxylin and eosin (H&E) as well as IHC stain for hCD45+ cells in primary patient BM (left column) and corresponding NSGS tissues (scale bars for low-power field: 200 μm, original magnification ×100; scale bars for high-power field: 50 μm, original magnification ×1600). (H) Flow cytometric analysis for hematopoietic stem and progenitor compartments of BM, spleen, liver, lung, and PB from the same NSGS-recipient mouse (the percentages represent the percent of cells within hCD45+ cells). CMP, common myeloid progenitor; FACS, fluorescence-activated cell sorting; GMP, granulocyte/macrophage progenitor; HSC, hematopoietic stem cell; LMPP, lymphoid-primed multipotent progenitor; MEP, megakaryocyte/erythroid progenitor; MPP, multipotent progenitor.

Robust engraftment of CMML patient samples into NSGS mice. (A) Schema of method for engraftment of human CD34+ (hCD34+) cells from the BM of CMML patients into sublethally irradiated NSGS mice and serial transplantation. Each patient sample was injected into 1 NSGS-recipient mouse. (B) Clinical and genetic characteristics of CMML patient samples successfully engrafted along with number of cells transplanted per recipient mouse and chimerism of hCD45+ cells in BM and spleen of NSGS mice, French-American-British (FAB) classification. (C) Kaplan-Meier survival curve of recipient NSGS mice engrafted with CD34+ cells from CMML patients (n = 8). (D) White blood cell (WBC) count, hemoglobin (Hb), and platelet (PLT) count of recipient NSGS mice at initial bleed (2 weeks posttransplantation) and at time of moribund state (the mean value is represented by the line inside the box ± standard deviation represented by lines above and below the box; *P < .05, **P < .01; number of individual mice is represented in the figure). (E) Cytomorphology of primary patient PB and BM (top) and corresponding NSGS-recipient mouse BM xenografted from the same patient (bottom). Black arrows indicate dysplastic neutrophils; red arrow indicates binucleated erythroid precursor; scale: 50 μm. (F) Immunophenotype of a representative CMML patient BM (left column) and tissues from a corresponding recipient mouse xenografted from the same patient (the percentages listed represent the percent of cells within the gate described above each plot). (G) Hematoxylin and eosin (H&E) as well as IHC stain for hCD45+ cells in primary patient BM (left column) and corresponding NSGS tissues (scale bars for low-power field: 200 μm, original magnification ×100; scale bars for high-power field: 50 μm, original magnification ×1600). (H) Flow cytometric analysis for hematopoietic stem and progenitor compartments of BM, spleen, liver, lung, and PB from the same NSGS-recipient mouse (the percentages represent the percent of cells within hCD45+ cells). CMP, common myeloid progenitor; FACS, fluorescence-activated cell sorting; GMP, granulocyte/macrophage progenitor; HSC, hematopoietic stem cell; LMPP, lymphoid-primed multipotent progenitor; MEP, megakaryocyte/erythroid progenitor; MPP, multipotent progenitor.

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